Table 1. Percentage of sequences obtained by PCR-based analysis and by rRNA-seq at the two sampling times and ratio of the two values pairs (% by PCR/% by rRNA-seq).

Phylum	Day 154 sampling			Day 189 sampling
	% (by PCR)	% (by RNA)	Ratio by PCR/by RNA	% (by PCR)	% (by RNA)	Ratio by PCR/by RNA
Acidobacteria	1.877	0.084	22.256	3.062	0.053	57.819
Actinobacteria	4.530	0.015	308.903	13.604	0.046	298.464
Bacteroidetes	1.359	0.151	9.020	1.590	0.399	3.989
Armatimonadetes	0.302	5.697	0.053	0.412	6.652	0.062
Chlorobi	23.598	2.595	9.093	10.984	1.648	6.662
Chloroflexi	2.071	0.867	2.387	1.914	0.978	1.953
Firmicutes	0.669	0.008	81.510	0.383	0.027	14.079
Gemmatimonadetes	4.659	0.206	22.612	6.302	0.177	35.642
Planctomycetes	24.892	87.362	0.285	14.694	86.459	0.170
Proteobacteria	34.060	2.705	12.593	43.463	3.301	13.166
Candidate Division BRC1	0.388	0.042	9.233	0.736	0.023	32.872
Candidate Division TM7	0.863	0.006	144.614	0.942	0.030	31.799

Only phyla for which a minimum of 10 sequences were available are reported in the table. Ratios resulting in values <1 are highlighted in boldface.