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. 2016 Aug 31;6:32189. doi: 10.1038/srep32189

Figure 1. Identification and characterization of the long non-coding RNA Lnc_ASNR in cancer.

Figure 1

(A) Expression of Lnc_ASNR in four types of paired tumor tissue samples by microarray. N represents normal tissue samples, and C represents cancer tissue samples. (B) Genomic location of Lnc_ASNR relative to upstream and downstream genes. (C) Northern blots of the Lnc_ASNR and GAPDH transcripts. (D) Quantification of Lnc_ASNR expression in fractionated RKO and HCT116 cell lysates by qRT-PCR. U1 RNA serves as a positive control for nuclear gene expression, and GAPDH and β-actin serve as controls for cytoplasmic gene expression. Error bars represent SEM, n = 3. (E) qRT-PCR of Lnc_ASNR and its host and neighboring genes in RKO cells after knockdown of Lnc_ASNR by three different siRNAs. (F) qRT-PCR of Lnc_ASNR and its host and neighboring genes in HCT116 cells after transfection with the Lnc_ASNR expression vector.