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. 2016 Aug 31;6:32249. doi: 10.1038/srep32249

Figure 1. Preparation of datasets for analysis.

Figure 1

(A) Data filtering and gene expression analysis pipeline. (B) Mean correlation coefficient between replicates within the samples increases after filtering and subsampling (Pearson r, 2-tailed; n = 8; p-values by ANOVA with posthoc LSD). (C) On average over 95% of the filtered reads aligned to transcripts across cell types, with less than 5% percent aligning to introns and intergenic regions (n = 8, mean ± SEM of basepairs aligned to transcripts or introns/intergenic regions shown as percent of total aligned basepairs; alignment percent determined by Picard module RnaSeqMetrics).