Table 2. Independent maintenance of SGI1, mutant derivatives and IncA/C plasmid R55 in S. Agona strain 959SA97.
| Bacterial generation no. | SGI1WT | SGI1Δint | SGI1ΔsgiAT | IncA/C R55 | |
|---|---|---|---|---|---|
| CFU/ml | |||||
| phenotypic detection§ | 9 | 3.05 1010 (1.60 1010)£ | 3.90 1010 (1.95 1010) | 7.30 1010 (4.15 1010) | ND |
| 234 | 2.39 1010 (2.09 1010) | 1.81 1010 (1.31 1010) | 1.98 1010 (2.29 1010) | ND | |
| 351 | 1.24 1010 (1.78 1010) | 1.60 1010 (1.00 1010) | 1.98 1010 (9.25 109) | ND | |
| Molecular detection¥ | 18 | 100/100 | ND | 100/100 | 97/100 |
ND, not determined.
§The presence of SGI1 or mutant derivatives was monitored by plating serial dilutions on LB agar with or without kanamycin (resistance conferred by SGI1) during 351 bacterial generations as performed according to Kiss et al.7.
¥Bacteria carrying SGI1 or mutant derivatives and total bacteria were scored as the number of CFU/ml and are indicated in uppercase and lowercase (in parentheses) numbers, respectively.
£After 18 generations, dilutions were plated on LB agar. After O/N cultures at 37 °C, 100 CFUs were tested by PCR for the presence of SGI1, mutant derivatives or the IncA/C plasmid R55. The maintenance of R55 was assessed in S. Agona strain 959SA97ΔSGI1.