Figure 2. APBs detection in the different cell lines.

(A) Immunofluorescence and fluorescent in situ hybridization (IF-FISH) staining for different cell lines. The PML bodies are labelled red. The telomeric DNA was labelled with (5′-TTAGGG-3′)₃ probes (green). The nuclei were stained with DAPI (blue). The white arrowheads indicated PML and telomeric DNA co-localization. (B) Statistical quantification of (A), it indicated that the percentage of cells with more than three APBs. The error bars represented the standard error of three distinct experiments. *P < 0.05; **P < 0.01; ***P < 0.001; N.S., not significant. (C) IF-FISH staining. The PML bodies were labelled green. The telomeric DNA was labelled with (5′-CCCTAA-3′)₃ probes (red). The nuclei were stained with DAPI (blue). The white arrowheads indicated PML and telomeric DNA co-localization. (D) Statistical quantification of (C), it indicated that the percentage of cells with more than three APBs. The error bars represent the standard error of three distinct experiments. **P < 0.01; ***P < 0.001. ΔT, TPP1^ΔOBRD; shV, shRNA empty vector; shA, ATRX shRNA2; shD1, DAXX shRNA1; shD2, DAXX shRNA2; D-KO 1, D-KO 2 and D-KO 3 indicated the three DAXX knock out clones.