Table 1.
Complexes investigated.
| PDB | Protein/peptide | Ref. | KD | ΔGbind | Techn. |
|---|---|---|---|---|---|
| 1CKAA:B | C-Crk N-terminal SH3 domain | 1 | 1.90E–6 | −31.84 | TF |
| C3G peptide | |||||
| 1D4TA:B | T cell signal transduction molecule SAP | 2 | 6.50E–7 | −35.26 | FP |
| Signaling lymphocytic activation molecule | |||||
| 1MFGA:B | Erb-B2 interacting protein | 3 | 5.00E–5 | −24.51 | ITC |
| Erb-B2 carboxyl-terminal fragment | |||||
| 1PZ5AB:C | Antibody SYA/J6 | 4 | 4.00E–6 | −30.76 | ITC |
| MDWNMHAA peptide | |||||
| 1SE0A:B | Apoptosis 1 inhibitor | 5 | 7.60E–8 | −39.89 | ITC |
| Cell death protein Grim | |||||
| 1T4FM:P | MDM2 | 6 | 8.00E–8 | −40.44 | F |
| Peptidomimetic p53 | |||||
| 1T7RA:B | Androgen receptor | 7 | 1.10E–6 | −33.96 | SPR |
| FxxLF motif peptide | |||||
| 1TW6B:D | Baculoviral IAP repeat-containing protein 7 | 8 | 3.00E–8 | −42.87 | FP |
| Diablo homolog, mitochondrial | |||||
| 1W9EB:S | Syntenin 1 | 9 | 1.00E–3 | −17.38 | CSP |
| TNEFYF peptide | |||||
| 1X2RA:B | Kelch-like ECH-associated protein 1 | 10 | 1.81E–7 | −38.42 | ITC |
| Nuclear factor erythroid 2 related factor 2 | |||||
| 2AK5AB:D | Rho guanine nucleotide exchange factor 7 | 11 | 1.40E–5 | −27.01 | ITC |
| 8-residue peptide from CBL-B | |||||
| 2B9HA:C | Mitogen-activated protein kinase FUS3 | 12 | 8.00E–8 | −40.44 | FP |
| Serine/threonine-protein kinase STE7 | |||||
| 2CCHAB:E | Cell division protein kinase 2/cyclin A2 | 13 | 2.03E–8 | −43.84 | CD |
| Cell division control protein 6 homolog | |||||
| 2FOJA:B | Ubiquitin carboxyl-terminal hydrolase 7 | 14 | 2.10E–5 | −26.66 | TF |
| p53 peptide 364-367 | |||||
| 2HO2A:B | FE65 WW | 15 | 1.16E–4 | −22.28 | ITC |
| Mena Peptide 10 | |||||
| 2HPLA:B | PUB domain of mouse PNGase | 16 | 3.60E–6 | −31.02 | ITC |
| C-terminal of mouse p97/VCP | |||||
| 2O9VA:B | Src homology 3 (SH3) domain | 17 | 2.88E–4 | −20.18 | F |
| Paxillin | |||||
| 2R7GA:B | Retinoblastoma-associated protein | 18 | 9.00E–7 | −33.30 | ITC |
| Early E1A 32 kDa protein | |||||
| 3D1EA:P | DNA polymerase III subunit beta | 19 | 1.42E–6 | −33.32 | ITC |
| decamer from polymerase II C-terminal |
In each complex, the protein is underlined. The values of KD and ΔGbind are expressed in molar and kJ/mol, respectively. The techniques in the last column are: TF, Tryptophan Fluorescence; FP, Fluorescence Polarization; ITC, Isothermal Titration Calorimetry; F, Fluorescence; SPR, Surface Plasmon Resonance; CSP, Chemical Shift Perturbation; CD, Circular Dichroism.