Table 2.

Germination of wild-type and T₁ transgenic seeds of tobacco on MS basal medium amended with 150 and 200 mM NaCl for salt stress tolerance.

Conc. of NaCl used (mM)	Total no. of seeds inoculated	No. of seeds germinated
		WT	A line	D line	E line	F line	I line
150	50	23	45	48	48	46	49
200	50	0	40	43	42	41	45

Data are average of percent seed germination in control and TopoII transgenic tobacco lines. The experiment was repeated thrice with fifty T₁ seeds of TopoII expressing transgenic lines in each experiment.