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. 2016 Aug 31;7:282. doi: 10.3389/fphar.2016.00282

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Copyright © 2016 Czemplik, Mierziak, Szopa and Kulma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression levels of genes involved in the apoptosis of MCF-7 cells. The mRNA level of bcl-2 (B-cell CLL/lymphoma 2), bax (BCL2-associated X protein), p53, mdm-2 (MDM2 proto-oncogene), cas 7 (caspase 7), cas 8 (caspase 8), cas 9 (caspase 9) and annexin V(anex) in MCF-7 cells treated with Linola flax straw extracts (Lin.1, Lin.2, Lin.3) at 6 h (A), 12 h (B), and 24 h (C) was obtained from the real-time RT-PCR analysis. GAPDH was used as a reference gene and the transcript levels were normalized to those of the control non-treated cells (C = 1; not presented in the figure). Data represent the mean ± standard deviations from three independent experiments.