Table 1.
Information for the studied markers in adiponectin (ADIPOQ) and adiponectin receptor 1 (ADIPOR1) genes.
| Gene name (SNP ID) | Chromosome location (base change) | Forward primer Reverse primer |
PCR program (35 cycles) | PCR size (bp) | Restriction enzyme, incubation temperature | Alleles: RFLP size (bp) |
|---|---|---|---|---|---|---|
| ADIPOQ (rs2241766) | 3q27.3; Exon 2; (T > G) |
5′-GAAGTAGACTCTGCTGAGATGG-3′ 5′-TATCAGTGTAGGAGGTCTGTGATG-3′ |
93 °C 45 s,64 °C 30s, 72 °C 45 s |
372 |
SmaI, 30 °C |
Allele T: 372 Allele G: 216 + 156 |
| ADIPOR1 (rs2275738) | 1q32.1; Intron 1; (C > T) |
5′-TTTGTGGGAAGACTCTGGCTGGT-3′ 5′-TTAGTGAGGTTCTGGGTAAAGGTT GACATT-3′ |
93 °C 45 s,64 °C 30s, 72 °C 45 s |
300 |
RseI, 37 °C |
Allele T: 300 Allele C: 180 + 120 |