Table 1. GLM results of the effects of simulated climate change and microorganism exposure and their interaction on mussel toxicity, immunobiological status, oxidative metabolism, gill function and cellular energy status in the green mussel Perna viridis.
| Parameter | Climate change | Microorganism exposure | Climate change + Microorganism exposure |
|---|---|---|---|
| Toxicity | |||
| Toxin-pathogen load score | F3 = 1.057, P = 0.369 | F3 = 1.760, P = 0.157 | F9 = 1.953, P = 0.048 |
| PST | F3 = 5.155, P = 0.003 | F2 = 4.650, P = 0.013 | F6 = 5.573, P = 0.000 |
| Vibrio (RT-PCR) | F3 = 0.299, P = 0.826 | F2 = 0.332, P = 0.718 | F5 = 0.412, P = 0.840 |
| Immunobiological status | |||
| Neutral red retention | F3 = 14.344, P = 0.000 | F3 = 13.593, P = 0.000 | F9 = 3.476, P = 0.001 |
| Oxidative metabolism | |||
| O2 uptake Gill function | F3 = 1.860, P = 0.138 | F3 = 10.422, P = 0.000 | F9 = 2.694, P = 0.006 |
| Na+/K+-ATPase | F3 = 1.223, P = 0.303 | F3 = 1.169, P = 0.323 | F9 = 1.985, P = 0.044 |
| Cellular energy status | |||
| ATP | F3 = 1.096, P = 0.352 | F3 = 31.410, P = 0.000 | F9 = 9.886, P = 0.000 |
| ADP | F3 = 1.789, P = 0.151 | F3 = 17.486, P = 0.000 | F9 = 5.860, P = 0.000 |
| AMP | F3 = 3.181, P = 0.025 | F3 = 76.915, P = 0.000 | F9 = 4.518, P = 0.000 |
| AEC | F3 = 2.757, P = 0.044 | F3 = 60.820, P = 0.000 | F9 = 7.311, P = 0.000 |
| TAN | F3 = 2.091, P = 0.103 | F3 = 35.782, P = 0.000 | F9 = 4.024, P = 0.000 |
| Glucose | F3 = 2.544, P = 0.057 | F3 = 99.367, P = 0.000 | F9 = 1.314, P = 0.232 |
| Glycogen | F3 = 2.404, P = 0.069 | F3 = 13.537, P = 0.000 | F9 = 1.753, P = 0.080 |
Detailed test results for statistically significant effects of simulated climate change, and/or microorganism exposure are shown in Tables 2 and 3. F-values are given with degrees of freedom in subscript. Significant values (P < 0.05) are highlighted in bold. AEC, adenylate energy change. TAN, total adenylate nucleotides.