Table 3. Parameter estimates and Tukey post-hoc test results of the effects of microorganism exposure (Alexandrium, Vibrio and Alexandrium + Vibrio) on mussel toxicity, immunobiological status, oxidative metabolism and cellular energy status in the green mussel Perna viridis.
| Parameter | Alexandrium | Vibrio | Alexandrium + Vibrio |
|---|---|---|---|
| Toxicity | |||
| PST (μg STX eq kg−1) | 3.587, T = 2.88, P = 0.015 | NA | 0.269, T = 0.09, P = 0.996 |
| Immunobiological status | |||
| Neutral red retention (min) | 47.076, T = 5.148, P = 0.001 | 39.241, T = 6.112, P = 0.000 | 68.772, T = 3.691, P = 0.038 |
| Oxidative metabolism | |||
| O2 uptake (μmol O2 h−1 g−1) | 6.923, T = 2.114, P = 0.012 | 10.419, T = 5.564, P = 0.000 | 6. 602, T = 2.082, P = 0.134 |
| Cellular energy status | |||
| ATP (μmol g−1) | 0.866, T = 2.46, P = 0.069 | 1.372, T = 7.38, P = 0.000 | 1.249, T = 4.38, P = 0.000 |
| ADP(μmol g−1) | 1.002, T = 1.47, P = 0.458 | 0.757, T = 6.73, P = 0.000 | 1.05, T = 0.90, P = 0.806 |
| AMP(μmol g−1) | 0.907, T = 3.47, P = 0.004 | 0.519, T = 2.01, P = 0.186 | 1.44, T = 12.29, P = 0.000 |
| AEC | 0.507, T = 3.63, P = 0.002 | 0.662, T = 7.25, P = 0.000 | 0.488, T = 5.65, P = 0.000 |
| TAN | 2.775, T = 0.13, P = 0.999 | 2.648, T = 1.05, P = 0.723 | 3.739, T = 8.50, P = 0.000 |
| Glucose (μmol g−1) | 1.113, T = 12.01, P = 0.000 | 2.855, T = 13.17, P = 0.000 | 0.721, T = 14.78, P = 0.000 |
| Glycogen (μmol g−1) | 98.006, T = 3.04, P = 0.014 | 59.093, T = 6.27, P = 0.000 | 123.374, T = 2.25, P = 0.113 |
Results shown only for Parameters with statistically significant effects (Table 1).
T-values are given. Significant values (P < 0.05) are highlighted in bold. AEC, adenylate energy change. TAN, total adenylate nucleotides.