Figure 8.

Calcium imaging analysis of electrically evoked signal propagation in mPFC. (A) Representative DAPI stained horizontally sectioned brain slice of mPFC, showing the (inset indicated by *) region of interest studied with Fluo-4AM calcium imaging. The stimulating electrode was positioned within the white matter that projects into the mPFC. (B) Calcium signal responses were calculated normalized to baseline level (ΔF/F₀) in response to electrical stimulation. Image sequences were sampled (100 frames or 20 s) to capture calcium measures before and after electrical stimulation. (C) Representative image of Fluo-4AM imaging of mPFC. Electrical stimulation through bipolar stimulating electrode placed in the white matter evoked propagation of current through cortical layers. The pseudo-colored image illustrates the intensity of the current spread through the cortex.