Table 1. The experimental design for flow cytometric evaluation.
All antibody staining, except (*), was performed prior to overnight (o/n) permeablization. (*) indicates staining was performed after o/n permeablization.
| Group | Marker | Clone | Tag |
|---|---|---|---|
| 1 | anti-mouse CD3 | 145-2C11 | eFluor450 |
| anti-mouse CD4 | GK1.5 | PE-Cy7 | |
| anti-mouse CD8 | 53-6.7 | FITC | |
| anti-mouse/human CD44 | IM7 | APC | |
| anti-mouse/rat FoxP3 | FJK-16s | PE | |
| 2 | anti-mouse CD3 | 145-2C11 | PE-Cy7 |
| anti-mouse CD11b | M1/70 | FITC | |
| anti-mouse CD11c | N418 | PE | |
| anti-mouse CD45 | 30-F11 | eFluor450 | |
| anti-mouse Ly-6G (Gr1) | RB6-8C5 | APC | |
| 3 | anti-mouse CD3 | 145-2C11 | eFluor450 |
| anti-mouse CD4 | GK1.5 | PE-Cy7 | |
| anti-mouse CD8 | 53-6.7 | APC | |
| anti-mouse IFN-γ | XMG1.2 | FITC | |
| anti-mouse/rat IL-17A | eBio17B7 | PE |