Figure 5.
CPD is repaired slower in heterochromatin than that in euchromatin. OSU-2 cells were UV irradiated at 10 J/m2 and cultured for 0, 8 and 24h. The cells were fixed and subjected to the ChIP assay with either anti-H3K9ac or anti-H3K9me3 antibody. The recovered DNA was subjected to the ISB analysis to quantitate CPD. ssDNA detection was used as the loading control (A). The intensity of each band was quantitated, and relative amounts of CPD were calculated by normalizing to ssDNA first, and then to 0h (B). N = 3; Bar: SD. **P < 0.01 compared to H3K9ac-IP samples at the same time point.