Figure 4.
Microglial elimination in young 5xfAD mice does not affect amyloid-β pathology. One and a half-month-old 5xfAD mice were treated with PLX3397 or control chow for 28 days. (A) Experimental design. (B and C) Representative 10× immunofluorescent IBA1 and 6E10 images of the hippocampus and amygdala. (D) Quantification of microglial cells shows a significant reduction in the 5xfAD + PLX3397 group compared to the 5xfAD group in the hippocampus and amygdala (two-tailed unpaired t-test, P < 0.0001). (E and F) Levels of amyloid-β species in detergent-soluble fractions are unchanged with the elimination of microglia. Levels of amyloid-β1–38 in the detergent-soluble fraction were below detection threshold. Statistical significance is denoted by *P < 0.05. Error bars indicate SEM (n = 4/group). FOV = field of view.