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. 2016 Feb 26;139(4):1265–1281. doi: 10.1093/brain/aww016

Figure 6.

Figure 6

Modest changes in Alzheimer's disease-related genes with microglial elimination. Transcript levels for Alzheimer's disease-related genes were analysed using NanoString nCounter platform and immunoblots were performed to assess components of APP processing. (A) Alzheimer's disease-related gene transcript levels in all four experimental groups. Symbols denote significant differences between groups (P < 0.05): *Control versus PLX3397; †Control versus 5xfAD; #PLX3397 versus 5xfAD + PLX3397; ϕ5xfAD versus 5xfAD + PLX3397. (B and C) Immunoblotting for full-length APP, C99 and C83 showed significantly increased levels of each protein in 5xfAD mice compared to Control (via two-way ANOVA; P < 0.0001) and in 5xfAD + PLX3397 mice compared to PLX3397 (via two-way ANOVA; P < 0.0001), but showed no effect of microglial elimination in 5xfAD mice. Statistical significance is denoted by *P < 0.05. Error bars indicate SEM (n = 3–6/group).