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. 2016 Aug 31;6:32416. doi: 10.1038/srep32416

Figure 5. Overexpression of PBEF by viral transduction reduces mtDNA loss after glutamate and OGD stimulations.

Figure 5

(A,B) Agarose gel images of mtDNA and nucDNA. Neurons without and with AAV infection were treated with 3 μM glutamate together with 0.3 μM glycine for 24 h (A) or were exposed to 1 h OGD followed by 24 h reperfusion (B). (C,D) Quantification of mtDNA amount after glutamate (C) and OGD (D) stimulations. The data were presented as the ratio of mtDNA/nucDNA and normalized to the control condition using nucDNA as an internal control. Notice that the overexpression of PBEF by viral infection prevented the glutamate-induced decrease of mtDNA content. Data are shown as mean ± SE; n = 3 independent experiments. (C) **P < 0.01 versus Glu, ANOVA test. (D) **P < 0.01, *P < 0.05 versus OGD, ANOVA test.