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. 2016 Aug 31;36(4):e00375. doi: 10.1042/BSR20160243

Figure 4. Experimental validation of the predicted targets in macrophage cells.

Figure 4

The WT construct had the 3’ UTR fragment of the target gene containing the predicted miRNA binding sites inserted within the Renilla luciferase 3’ UTR. The mutant (MT1 and MT2) construct was identical with the WT construct except that it had a point substitution disrupting binding site pairing to each miRNA seed. The bar graphs show the luciferase activity after the reporter plasmids were transfected into macrophage cells. Statistically significant differences compared with WT construct are visualized as *P≤0.05, #P≤0.01.