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. 2016 Aug 30;9(1):472. doi: 10.1186/s13071-016-1752-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Differential leukocyte counts in peripheral blood leukocytes profile in BALB/c mice immunized with the multicomponent LBSap vaccine, Leish-Tec® or Leishmune®. The x-axis displays the times at which the assays were conducted (before first vaccination [BV], 15 days after third saline [15^ASaline] or vaccination [15^AVac] and 30 days after experimental L. infantum challenge [30^AChal]) in the different analyzed groups (n = 5 mice/group in two independent batches) (Control [□]; Leish-Tec® []; Leishmune® []; and LBSap [killed L. braziliensis vaccine plus saponin; ■]). The y-axis represents the mean values (with standard deviations) of the frequencies of neutrophils, monocytes, eosinophils, and lymphocytes based on the hemogram. Connecting lines represent significant intra-group differences (P < 0.05) at the different time points. Inter-group differences at the same time point are marked by letters (LM: Leishmune® group and LB: LBSap group)

Inline graphic — Differential leukocyte counts in peripheral blood leukocytes profile in BALB/c mice immunized with the multicomponent LBSap vaccine, Leish-Tec® or Leishmune®. The x-axis displays the times at which the assays were conducted (before first vaccination [BV], 15 days after third saline [15^ASaline] or vaccination [15^AVac] and 30 days after experimental L. infantum challenge [30^AChal]) in the different analyzed groups (n = 5 mice/group in two independent batches) (Control [□]; Leish-Tec® []; Leishmune® []; and LBSap [killed L. braziliensis vaccine plus saponin; ■]). The y-axis represents the mean values (with standard deviations) of the frequencies of neutrophils, monocytes, eosinophils, and lymphocytes based on the hemogram. Connecting lines represent significant intra-group differences (P < 0.05) at the different time points. Inter-group differences at the same time point are marked by letters (LM: Leishmune® group and LB: LBSap group)