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. 2016 Aug 30;17(1):690. doi: 10.1186/s12864-016-3031-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 11 — PCR amplification of genes from scaffolds 13 and 20 in isolates 14H1-11A and CIRAD86. PCR amplification was done for three genes encoding hypothetical proteins on scaffolds 13 and 20, as well as β-tubulin as a positive control. Genomic DNA from isolates 14H1-11A (used in this RNA-Seq analysis) and CIRAD86 (genome sequence publicly available) was used as a template for PCR assays, with water used as a negative control. Quick-Load 100 bp DNA ladder (NEB) was used as a molecular weight marker. Red rectangles mark the expected product size for each assay