Fig. 4.

Expression of single perforin allele is sufficient to induce impaired locomotor activity during immune-mediated BBB disruption. Locomotor activity was assessed in the open field behavior test for uninfected or post-PIFS perforin−/−, perforin+/−, and perforin+/+ mice. a–c Representative activity box tracking images are shown for uninfected a perforin−/− mice (n = 6), b perforin+/− mice (n = 8), and c perforin+/+ mice (n = 5). d, e Representative activity box tracing images are shown for post-PIFS d perforin−/− mice (n = 8), e perforin+/− mice (n = 6), and f perforin+/+ mice (n = 5). g Ambulatory horizontal distance traveled was calculated over 60 min for each genotype for both uninfected (dark bars) and post-PIFS (white bars). h The total resting units, time spent with no movement at all, was also calculated over 60 min for each genotype for both uninfected (dark bars) and post-PIFS (white bars). Significance was determined using a two-way ANOVA with Tukey post hoc test. p < 0.05 considered statistically significant