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Differentiation of dental papilla mesenchymal cells and odontoblast marker gene expression. (a, a′) Alkaline phosphatase (ALP) and (b, b′) Alizarin red S (Alizarin) staining of iMDP-3 cells on days 7 and 14 after differentiation induction. Messenger RNA of (c) Dmp1, (d) Dspp and (e) Runx2 in iMDP-3 cells after odontoblastic induction. Cell numbers (f) on days 7 and 14 after differentiation induction at the given time periods. *P<0.05 with significant difference versus day 0
