Table 1.
Comparison of hydrogenase activity in wt (CC-124 and D66) and Fd-HydA transformants (1D4 and P6)
| D66 (HydA) | CC-124 (HydA) | 1D4 (Fd-HydA) | P6 (Fd-HydA) | |
|---|---|---|---|---|
| Photosynthetic H2 production [µmol(H2) mg(chl)−1 h−1] | 8.34 ± 1.4 | 21.65 ± 1.5 | 0.91 ± 0.027 | 6.06 ± 0.538 |
| Chemical H2 production [µmol(H2) mg(chl)−1 h−1] | 37.68 ± 8 | 104.6 ± 14.4 | 4.5 ± 0.55 | 26.9 ± 0.298 |
| Amount of enzyme [ng(enzyme) mg(chl)−1] | 628 | 1743 | 21 | 128 |
| Amount of enzyme [µmol(enzyme) mg(chl)−1] | 1.31 × 10−5 | 3.63 × 10−5 | 0.35 × 10−6 | 2.14 × 10−6 |
| Photosynthetic H2 production rate standardized for hydrogenase amount (PHPRH) [µmol(H2) µmol(enzyme)−1 min−1] | 10,622 | 9931 | 42,909 | 47,236 |
| Ratio of PHPRH (transformant/wt average) | ~1 | ~1 | 4.2 | 4.6 |
All of the experiments were performed in biological triplicates