Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 30;17(1):21. doi: 10.1186/s12867-016-0072-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 10 — Polysome profile analysis revealed that eIF4E3_A associates with translation intiation complexes and light polysome fractions. GFP-eIF4E1 (a) and GFP-eIF4E3_A (c) stably expressed in HEK293 cells are distributed along polysome profiles similarly to endogenous eIF4E1 (b). To evaluate possible influence of a GFP-fusion tag, a polysome profile analysis from HEK293 cells stably expressing GFP alone was performed (b). Western blots of the first nine fractions were probed either with anti-eIF4E1 antibody (a, b) or anti-GFP antibody (b, c). Highest amounts of GFP-eIF4E1, GFP-eIF4E3_A and endogenous eIF4E1 were detected from the end of the loading peak to the light polysomes, whereas GFP protein alone was detected in the early loading peak exclusively. Loading peak (≈fractions 1–3); 40S, 60S and 80S (≈fractions 4–6 in a, b and 3–5 in c); light and heavy polysomes (≈fractions 7–10 in a, b and 6–10 in c)