Fig. 2.

GFP-eIF4E1 fusion protein is capable to bind to m⁷GTP agarose. HEK293 cells were lysed (INPUT) and the lysate was incubated with the m⁷GTP-agarose. Western blot was developed with anti-eIF4E1 antibody, which clearly shows that both endogenous eIF4E1 and its GFP-eIF4E1 fusion counterpart retain their ability to bind the m⁷G cap. Actin was used as a control of a sufficient washing of the m⁷GTP affinity resin