Fig. 6.

eIF4E1_3 and eIF4E3_A isoforms are less prone to form SGs than the prototypical eIF4E1_1. eIF4E1_1, eIF4E1_3, and eIF4E3_A proteins were ectopically produced in fusion with GFP from the same vector in U2OS cells. Nineteen hours post-transfection, the cells were treated with 1 mM sodium arsenite for 40 min, and those forming SGs were counted and plotted as a fraction of all transfected cells. Error bars indicate differences among three independent experiments, in which approximately 100 of the transfected cells were assessed. We applied Chi square test to analyze differences between number of cells forming stress granules among all transfected cells expressing individual eIF4E proteins or a GFP control. GFP-eIF4E1_1, GFP-eIF4E1_3, GFP-eIF4E3_A were compared to control pGFP and to each other by post hoc Chi square test with Bonferroni correction for multiple testing. Exept the GFP-eIF4E1_3 x GFP-eIF4E3_A pair, all other differences were statistically significant (p values <0.0001, marked with asterisk)