Fig. 9.

Control immunoprecipitation experiment does not reveal any non-specific interaction between GFP and eIF4G or PABP: HEK293 cells transiently transfected with a control expression vector pEGFP-C1 were lysed 24 h post-transfection and the lysate was subjected to immunoprecipitation using GFP-Trap approach. Western blots were developed with anti-eIF4G, anti-PABP, anti-GFP and anti β-actin antibodies. The results clearly show that while all the proteins tested were present in the lysate, only GFP remained bound to the resin upon GFP-Trap immunoprecipitation