Table 1.

The eIF4E isoforms used throughout this study and primers used to clone the corresponding cDNA

Gene	Protein isoform	Primer sequence		Restriction site	Protein variant (Acc. No./GI)	Encoded by transcript variant (Acc. No./GI):
eIF4E1	1	fwd	CGAAGAAGATCTATGGCGACTGTCGAACCGG	BglII	NP_001959.1 GI:4503535	1	NM_001968.3 GI:194578905
		rev	GCGCGGTACCTTAAACAACAAACCTATTTTTAGTGGTGG	Acc65I
	3	fwd	TTGTCGACATGTTGGACCTGACCTCCCGC	SalI	NP_001124150.1 GI:194578907	3	NM_001130678.1 GI:194578906
		rev	GCGCGGTACCTTAAACAACAAACCTATTTTTAGTGGTGG	Acc65I
eIF4E2	A	fwd	GTGTCGACATGAACAACAAGTTCGACGCTTTGAAAGATG	SalI	NP_004837.1 GI:4757702	1	NM_004846.3 GI:545309374
		rev	GGGTACCTCATGGCACATTCAACCGCGGCTTC	Acc65I
	C	fwd	GTGTCGACATGAACAACAAGTTCGACGCTTTGAAAGATG	SalI	NP_001263265.1 GI:545309640	3	NM_001276336.1 GI:545309639
		rev	CCGGTACCTCACAATGTGATTTTTGTATTTCGAAAGC	Acc65I
	CRA_a	fwd	GTGTCGACATGAGTTTGAAAGATGATGAC	SalI	EAW71007.1 GI:119591413		not annotated yet
		rev	CCGGTACCTCACAATGTGATTTTTGTATTTCGAAAGC	Acc65I
eIF4E3	A*	fwd	CGGAGAAAATGGCGCTGCCC	X	NP_001128123.1 GI:197382708	1	NM_001134651.1 GI:197382707
		rev	ACTGTGGACGTGCTGTCCTTGG	X
	B	fwd	GTGTCGACATGAGAGGAGAGAGGCGACCACTTTG	SalI	NP_775495.1 GI:27659734	2	NM_173359.4 GI:197382627 NM_001134649.2 GI:544583504 NM_001134650.1 GI:197382663 NM_001282886.1 GI:544583491
					NP_001128121.1 GI:197382649	3
					NP_001128122.1 GI:197382664	4
		rev	CCGGTACCTTAGTGTTTTCCACGTCCACCTTCAAAAG	Acc65I	NP_001269815.1 GI:544583492	5

* Indicates primers used for the amplification of the 3′-incomplete coding sequence of eIF4E3_A from HEK293-cell cDNA; the complete ORF was obtained in subsequent cloning steps