Fig. 7.

Stat3 is a novel direct target of miR-125b, which is involved in ATO regulating EMT-Associated traits in chondrosarcoma cells. a ATO inhibits Stat3 in a time and dose-dependent manner. b The Stat3 3′UTR region containing the wild type or mutant binding site for miR-125b. c Dual-luciferase reporter assay of miR-125b with 3’UTR fragments (wild type or mutant) of human Stat3 (NM_003150.3). Western blotting analysis of Stat3 expression in miR-125b-overexpressing cells (d) and in cells post-miR-125b inhibitor transfection (e) or with cotransfection with si-Stat3 (f). g Knockdown of miR-125b blocked the ATO-induced inactivation of Stat3 in HCS-2/8 and OUMS-27 cells