Table 1.

Effects of several concentrations of NaCl on the nodulation of C. glauca plants inoculated with Frankia strains CcI3 and CeD.

Frankia strains	NaCl treatments (mM)		Nodulation kinetic (Days after inoculation)
			14	21	28	35	42	49	56	63
CcI3	0	Nodulation rate (%)	72.7	95.5	100	100	100	100	100	100
		Mean nodule number	4.4 ± 1.1	9.9 ± 1.7	27.9 ± 3.4	36.1 ± 4.2	40.4 ± 4.4	42.2 ± 4.1	44.1 ± 4.2	45.8 ± 4.2
	50	Nodulation rate (%)	36.4	59.1	86.4	86.4	90.9	90.9	90.9	95.5
		Mean nodule number	0.8 ± 0.3	3 ± 0.8	7.4 ± 1.4	9.6 ± 1.9	10.2 ± 2	11.7 ± 1.9	13.4 ± 2.2	15.3 ± 2.3
	100	Nodulation rate (%)	4.5	18.2	40.9	40.9	40.9	40.9	40.9	40.9
		Mean nodule number	0.05 ± 0.05	0.4 ± 0.2	2.4 ± 0.9	3.6 ± 1.3	3.7 ± 1.4	4.2 ± 1.6	4.5 ± 1.7	4.9 ± 1.8
	≥200	Nodulation rate (%)	0	0	0	0	0	0	0	0
		Mean nodule number	0	0	0	0	0	0	0	0
CeD	0	Nodulation rate (%)	50	90.9	100	100	100	100	100	100
		Mean nodule number	2.3 ± 0.7	11 ± 2	28 ± 3.3	37.1 ± 3.3	38.8 ± 3.3	44.8 ± 3.4	48.8 ± 3.5	50.3 ± 3.5
	50	Nodulation rate (%)	18.2	50	68.2	68.2	68.2	81.8	81.8	81.8
		Mean nodule number	0.5 ± 0.3	2.7 ± 0.8	7.1 ± 1.5	12.2 ± 2.5	13.5 ± 2.6	16.7 ± 2.8	18 ± 3	20 ± 3.2
	100	Nodulation rate (%)	4.5	9.1	22.7	40.9	40.9	59.1	63.6	68.2
		Mean nodule number	0.05 ± 0.05	0.1 ± 0.1	3 ± 1.2	4.6 ± 1.6	4.7 ± 1.6	7.9 ± 2.3	9.1 ± 2.4	9.7 ± 2.5
	200	Nodulation rate (%)	0	0	0	0	0	9.1	9.1	22.7
		Mean nodule number	0	0	0	0	0	0.5 ± 0.3	0.6 ± 0.4	0.8 ± 0.5
	≥300	Nodulation rate (%)	0	0	0	0	0	0	0	0
		Mean nodule number	0	0	0	0	0	0	0	0

Salt stress was first gradually applied then plants were inoculated separately with each Frankia strain. Nodulation rate is the percentage of plant nodulated and mean nodule number is the average number of nodule per plant. Values represent the mean ± standard deviation of plants used in each treatment (n = 22).