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Workflow of sequence search and validation. In all, 378 myosins from 35 classes (Odronitz and Kollmar et al., 2007) were taken as queries for a Jackhmmer search against the NR database. The hits were filtered for the presence of functional (ATP-binding) motifs to obtain 4,064 sequences. The hits were aligned using the indicated parameters in Clustal-W and a phylogenetic tree was constructed using RAxML.
