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. 2016 Jun 29;10:174–184. doi: 10.1016/j.ebiom.2016.06.040

Fig. 2.

Fig. 2

Effect of SR10171 and rosiglitazone on trabecular and cortical bone of lean and DIO C57BL/6 mice.

(a)–(c) Analysis of trabecular bone in proximal tibia of lean mice. (a) mCT renderings (upper panels) and measurements of trabecular parameters. Bar — 0.1 mm. (b) Dynamic histomorphometry of bone double-labeled with calcein. Microphotographs of entire proximal tibia labeled with calcein (upper panels) (magnification 5 ×; bar — 0.2 mm) and bone surface labeled with calcein (lower left panels) (magnification 40 ×; bar — 0.02 mm). Quantification of mineral apposition rate (MAR) and bone formation rate (BFR) (lower right panels). (c) Number of osteoblasts per bone perimeter (N.Ob/B.Pm), osteoclasts per bone perimeter (N.Oc/B.Pm), osteoblasts to osteoclasts ratio (N.Ob/N.Oc), and number of adipocytes (N.Ad) per region of interest (ROI). (d) Glucose tolerance in DIO mice at the end of experiment. (e)–(f) Analysis of trabecular bone in tibia of DIO mice. (e) mCT measurements of trabecular parameters. (f) MAR and BFR quantified from bone double-labeled with calcein. (g) mCT renderings of fat stained with osmium tetroxide (left panels, fat appears as yellow, bar — 1.0 mm) and its volumetric quantification (right panel). (h) Cortical bone of lean animals. (i) Cortical bone of DIO animals. Representative microphotographs of calcein-labeled endosteal and periosteal bone surface (upper panels) (magnification 5 ×; bar — 0.2 mm), histomorphometry of labeled periosteal bone perimeter (MS/BS) and osteocyte lacunae density (lower panels). TV — tissue volume; BV — bone volume; BV/TV — bone mass; Tb.N — trabecular number; Tb.Th — trabecular thickness. Data represent the mean ± SD (n = 3–7 per group). *P < 0.05; **P < 0.01; ***P < 0.001 One Way Anova.