Figure 7.

MMD1 Regulates the Expression of the Condensin Gene CAP-D3 in Meiocytes.

(A) Schematic depiction illustrating the subunit composition of condensin complexes in plants as described in the text.

(B) qRT-PCR analysis of condensin gene expression in wild-type and mmd1 male meiocytes. Data were normalized to ACTIN expression.

(C) CAP-D3 gene structure and the positions of primers.

(D) Validation of MMD1 antibody through analysis of nuclear proteins from core flower buds by protein gel blot analysis.

(E) ChIP-PCR analysis of MMD1 occupancy on CAP-D3 using wild-type inflorescences. Chromatin from leaves was used as a negative control.

(F) ChIP-PCR analysis of MMD1 occupancy on other condensin genes. Data are represented as mean ± se from three (in [B] and [E]) and four (in [F]) independent experiments. *P < 0.05 and **P < 0.01, two-tailed Student’s t test.