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Physical Interaction between MOF and OSK1.
(A) Yeast two-hybrid assay for interaction of MOF with OSK1. A schematic diagram of MOF and the truncations used is shown. The interactions were verified by the growth of yeast strains on the -Leu-Trp-His-Ade+X-Gal selection medium.
(B) BiFC assay for interaction between the MOF and OSK1 in rice protoplasts.
(C) Coimmunoprecipitation of MOF-HA and OSK1-FLAG based on anti-FLAG immunoprecipitation from transfected Nicotiana benthamiana leaves.
