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. 2016 Jul 6;28(8):1795–1814. doi: 10.1105/tpc.16.00310

Figure 1.

Figure 1.

P. japonicum Infecting Host Roots.

(A) Three-month-old P. japonicum. Bar = 1 cm.

(B) and (C) Interaction of P. japonicum root with Arabidopsis root (B) and rice root (C). Bars = 200 µm.

(D) and (E) Double-stained cross section of P. japonicum haustoria. Technovit 7200-embedded tissues were cross-sectioned at 5 weeks after host interaction and stained with safranin-O and Fast Green. P. japonicum penetrating Arabidopsis (D) and rice (E). Bar = 50 µm.

(F) Example of a haustorium at the immature stage at 3 dpi.

(G) Safranin-O-stained haustorium at 3 dpi. Note the vasculature connection has not yet been established.

(H) Haustorium at a later stage when the parasite is already tightly connected to the host rice root at 7 dpi.

(I) Safranin-O-stained haustorium at 7 dpi in rice interaction. The xylem bridge has been established. Bars = 250 µm in (F) to (I). pa, parasite; ho, host roots; xy, xylem bridge; px, plate xylem. Asterisks indicate haustoria. Arrows indicate the interface between a host and a parasite.