Figure 3.

YUC3 Is Upregulated during Haustorium Development.

(A) A maximum likelihood phylogenetic tree of Arabidopsis (At) and P. japonicum (Pj) YUC proteins. The bootstrap values are shown in percentage on the internal nodes.

(B) and (C) Expression levels of YUC genes measured by RT-qPCR. The values correspond to fold change compared with nontreated roots.

(B) YUC expression in 2-week-old P. japonicum root treated with host root exudate for 0.5, 1, 3, 24, and 48 h.

(C) YUC expression in host interaction. Haustorial region was excised after contact with rice roots for 1, 3, and 7 d postinfection (dpi). Values are shown in fold changes compared with the root without host interaction. All data show the average of three biological replicates with at least two technical replicates each. Error bars denote se. Asterisks indicate significant differences compared with mock treatment (B) and control samples (C) at α ≤ 0.05 by Student’s t test with equal variances.