Figure 4.

Overexpression of YUC3 in Transgenic P. japonicum Roots Shows an Auxin-Overproduction Phenotype.

(A) and (B) Control transgenic roots expressing CaMV 35S promoter upstream of the GFP gene (Pro35S:GFP) observed using bright-field (A) or fluorescence (B) microscopy.

(C) Nontransformed roots stained with safranin-O and cleared by chloral hydrate.

(D) Expression levels of YUC3 in nontransformed roots (control) and ProUB:PjYUC3 transformed root analyzed by RT-qPCR. Data are presented as the means with the se of two technical replicates of two biological replicates containing 5 to 10 roots in each experiment.

(E) to (G) Phenotype of transgenic roots overexpressing YUC3 observed under bright field (E) or fluorescence (F) microscopy. Transgenic root was stained with safranin-O and cleared with chloral hydrate (G). Asterisks denote lateral roots.

(H) Endogenous IAA levels in root tip tissues (2 mm form the root tips) overexpressing YUC3 (ProUB:PjYUC3) or empty vector (control). An asterisk indicates significant difference at α ≤ 0.05 by Student’s t test with equal variances (P value < 0.002). White bars = 2 mm, and black bars = 200 µm