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. 2016 Jul 6;28(8):1795–1814. doi: 10.1105/tpc.16.00310

Figure 5.

Figure 5.

Tissue Localization of YUC3 Expression during Haustorium Development.

(A) Transgenic roots carrying a YUC3 promoter-driven VENUS fluorescent protein gene fused with a nuclear targeting signal. CaMV 35S promoter-driven RFP was inserted as a visual marker (ProPjYUC3:3xVENUS-N7-Pro35S:RFP). Roots without haustorium-inducing treatment were observed under bright-field, RFP, and VENUS fluorescence detecting filter sets.

(B) and (C) Transverse sections of a root transformed with the YUC3 promoter driving the GUS reporter gene (ProPjYUC3:GUS). Nontreated root (B) and 24 h after rice root-exudate treatment (C).

(D) Time-lapse series of ProPjYUC3:3xVENUS-N7-Pro35S:RFP root showing the interaction of a P. japonicum transgenic root with Arabidopsis. Representative photographs are shown from time-lapse images taken every 30 min for 6 d. Bright-field and YFP filter photos were merged. The complete movies are shown in Supplemental Movies 2 to 4.

(E) Confocal microscopy photos of ProPjYUC3:3xVENUS-N7-Pro35S:RFP roots under bright-field (right), YFP filter (middle), and merged pictures (left) after 48 h of DMBQ treatment. Arrowheads point to YUC3 expression at haustorial hairs. Bars = 100 µm.