Fig. 6.

USP11 promotes LPA-LPA1 signaling. a. MLE12 cells were transfected with USP11-HA plasmid (0–2 μg) for 48 h, and then cells were treated with LPA (5 μM) for 10 min. Cell lysates were analyzed by immunoblotting with p-p38 MAPK, p38 MAPK, p-Erk1&2, Erk1&2, p-IκB, HA, and β-actin antibodies. Representative immunoblots were from at least three independent times. b. HBEpCs were transfected with USP11-HA plasmid for 48 h, and then cells were treated with LPA (1 μM) for 3 h. Cells were collected and IL-8 mRNA levels were examined by RT-realtime PCR. c. MLE12 cells were transfected with Usp11 shRNA for 72 h, and then cells were treated with LPA (5 μM) for 10 min. Cell lysates were analyzed by immunoblotting with p-Erk1&2, Erk1&2, and HA antibodies. Representative immunoblots were from at least three independent times. d. MLE12 cells were transfected with usp11 shRNA for 72 h, and then cells were treated with LPA (5 μM) for 6 h. Cell culture supernatants were collected and KC levels in the media were analyzed by Elisa. The expression of USP11 was determined by immunoblotting with USP11 and β-actin antibodies (insert). e. HBEpCs were transfected with USP11 shRNA for 72 h, and then cells were treated with LPA (1 μM) for 3 h. Cells were collected and IL-6 mRNA levels were analyzed by RT realtime PCR.