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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: RSC Adv. 2016 Mar 21;6(37):30895–30904. doi: 10.1039/C5RA26092B

Fig. 3.

Fig. 3

HSAF-Dependent ROS production led to fungal cell death. Histograms showed the fluorescence intensity of incubated with DCFH-DA. Cells treated with 3, 6 and 12 μg/mL HSAF for 1, 2 and 3 h (A). Changes in mitochondrial membrane potential. Cells were treated with DMSO, 3, 6 and 12 μg/mL HSAF for 3 h. Stained with DiOC6(3) and analyzed by flow cytometry (B). ROS scavenging agents reduces the antifungal activity of HSAF against C. albicans. Scavengers diminished the toxicity of HSAF by 4-fold for 0.5 mM AA and by 2-fold for 0.5 mM NAC and GSH. Log of CFU/ml remaining after drug exposure in the presence and absence of 0.5 mM Vc or TU at 12 μg/mL HSAF.