Table 4.
Statistical Analysis of Telomere Parameters by Neuroblastoma Cell Line
| SHEP Control | SHEP MYCN-Transfection | GIMEN Control | GIMEN MYCN-Transfection | P Value between SHEP Conditions* | P Value between GIMEN Conditions* | |
|---|---|---|---|---|---|---|
| Average MYCN expression per cell (RFU) | 1480.78 | 6962.54 | 1577.19 | 7839.49 | <.0001 | <.0001 |
| Average number of telomeres per nucleus | 41.8 | 74.9 | 85.7 | 120.3 | <.0001 | <.01 |
| Average number of aggregates per nucleus | 4.9 | 9.6 | 10.6 | 15.1 | <.05 | <.01 |
| Average percentage of cells per replicate with aggregates (%) | 71.3 | 100.0 | 80.7 | 100.0 | <.001 | <.01 |
| Average nuclear volume (μm3) | 1053.31 | 1262.03 | 1934.85 | 2225.43 | NS† | NS† |
| Average number of telomeres per nuclear volume | 0.040 | 0.060 | 0.044 | 0.054 | <.05 | <.05 |
| Average a/c ratio per cell | 3.34 | 6.34 | 6.32 | 11.32 | <.01 | <.05 |
| Average percentage of short telomeres per cell (%) | 15.1 | 30.5 | 24.7 | 39.1 | <.001 | <.01 |
| Average percentage of medium telomeres per cell (%) | 55.7 | 47.1 | 52.2 | 38.5 | <.05 | <.05 |
| Average percentage of long telomeres per cell (%) | 29.3 | 22.4 | 23.1 | 22.4 | <.05 | NS |
*
P value determined by unpaired t test.
†
Not significant.