Figure 9.

ChiX competes with RNA‐IN for Hfq‐binding in vivo. hfq⁻ cells containing the chromosomal IS 10 _1–339‐lac Z translational fusion (DBH299) were co‐transformed with a plasmid encoding Hfq^WT‐3xFLAG (pDH909) and a plasmid expressing ChiX (pDH765) or vector control (pDH763). Hfq was immunoprecipitated from cell lysates with ANTI‐FLAG ^® M2 magnetic beads. Total input RNA (10 μg) or RNA recovered from the IP (0.3 μg) was analyzed by Northern blot for ChiX and the 5S rRNA, or primer extension of RNA‐IN and lpp. Band intensities were quantified using ImageQuant.