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. 2016 Apr 22;5(7):e1170264. doi: 10.1080/2162402X.2016.1170264

Figure 3.

Figure 3.

HSP110DE9 hampers HSP110 release (A) Concentration of extracellular HSP110 in the supernatant of Lovo, HCT116 and SW480 transfected with a control GFP plasmid or a plasmid coding HSP110-GFP and measured by ELISA (n = 3) *p < 0.05 ; **p < 0.01. (B) ELISA quantification of HSP110 in the extracellular medium of SW480 transfected with a control GFP or HSP110DE9-GFP plasmid (n = 3) *p < 0.05. (C) Immunoblot analysis of HSP110 in the supernatant of HCT116, transfected with a HSP110-GFP plasmid with or without a plasmid coding HSP110DE9-GFP. (D) Flow cytometry analysis of HLA-DR (n = 6), CD163 (n = 6) and CD206 (n = 6) expression on macrophages derived from monocytes in the presence of supernatant from SW480 cells transfected with a control GFP, HSP110-GFP or a HSP110DE9-GFP plasmid. Left, data of all experiments; Right, representative data *p < 0.05; **p < 0.01; ***p < 0.001. (E) TNFa, and NO (nitrite) secreted by macrophages derived from monocytes in the presence of SW480 transfected as in C, and stimulated for 24 h with LPS (n = 4, *p < 0.05). (F) Percentage of proliferating allogeneic T cells after 3 d of culture with 104 macrophages derived from monocytes in the presence of supernatant from SW480 transfected with a control GFP or a HSP110DE9-GFP (n = 3). *p < 0.05.