Figure 1.

Human Oral Squamous Cell Cancers are inflammatory neoplasms. Single cell suspensions were obtained from human OSCC samples (n = 7) and healthy gingiva (n = 7), and analyzed by flow cytometry. (A) Gating strategy: after dead cells and doublets exclusion, nine subpopulations were identified within CD45⁺ cells: (a) CD15⁺CD11b⁺ (granulocytes), (b) CD14⁺CD11b⁺ (macrophages), (c) CD19⁺CD3⁻ (B cells), (d) CD56⁺CD3⁻ (Natural Killer cells), (e) CD3⁺CD4⁺ (CD4⁺ T cells), (f) CD3⁺CD8⁺ (CD8⁺ T cells), (g) CD3⁺CD4⁺CD25⁺FoxP3⁺CD127⁻ (Treg), (h) Lin-1neg (CD3⁻CD19⁻CD56⁻) HLA-DR⁺CD11c⁻ (pDC) and (i) Lin-1negCD-11c⁺HLA-DR⁺CD14⁻ (mDC). (B) Number of CD45⁺ cells per gram (g) of tissue (left panel), cell number of indicated subset per g of tissue (upper right panel) and percent of indicated subset within CD45⁺ cells (lower right panel) are presented. NS, non-statistical difference = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001.