Figure 4.

Adjuvants enhanced the therapeutic antitumor effects of intra-cheek vaccinations. C57BL/6 mice bearing intra-cheek TC-1-Luc tumors were ID or IC immunized at days 7–9–11 (arrow) with pVLP-E7 in the presence of adjuvants: CpG-ODN + Imiquimod (CpG/IMQ). As controls, one group of mice received CpG/IMQ alone and another received PBS. (A) Monitoring of tumor volume measured every 2–4 d; tumor-free rates are indicated in italic letters. (B) Kaplan–Meier curves showing tumor-free survival rates. Presented data are pooled from three separate experiments using 3–4 mice per group. *p < 0.05; ***p < 0.001; ****p < 0.0001.