Figure 2.

Phenotypic features of CD169⁺CD8⁺ T cells in the tumor-draining LN from CRC patients. The phenotypic characteristics of CD169⁺CD8⁺ T cells from fresh CRC tissues of peri-LN (PLN, A) or of distant-LN (DLN, B) were determined by flow cytometry. Data for perforin, granzyme B, TNF-α, and IFNγ were acquired in cells stimulated with Leukocyte Activation Cocktail (BD PharMingen, San Diego, CA) at 37°C for 5 h. Results are presented as means ± SEM of three separate experiments. Significant differences are indicated (*p < 0.05; **p < 0.01; ***p < 0.001).