Figure 7.

MYO3A serves as a key cancer cell intrinsic effector for macrophage-assisted cancer cell invasion via MIP-1β. TAMs-assisted cancer cell invasion via MIP-1β is dependent on upregulation of MYO3A gene within cancer cells. (A and B) Macrophage-induced matrix protrusion and invasion by cancer cells was abrogated upon siRNA-mediated silencing of MYO3A gene compare to scrambled in both C (mon-oculture cancer cells) and C + M (cancer cells co-cultured with macrophages). Bars represent mean invadopodia count/cell from 10 fields per experiment and mean invasive cell count ±SE (*p < 0.05.). All the experiments were done in triplicates and repeated at least thrice. Abbreviations—C: Respective cancer cells; C+M: Respective cancer cells co-cultured withmacrophages.