Figure 8.

Invasive breast adenocarcinoma MDA-MB-231 silenced with MYO3A exhibited diminished focal degradation of pericellular matrix, diminished invadopodia formation, compared to Scrambled control in presence of MIP-1β-Purified cytokine. Representative images from the in vitro matrix degradation assay. Cells (MDA-MB-231) Scr and silenced with MYO3A siRNA were seeded on Alexa Fluor 633 labeled gelatin (Red) in absence or presence of MIP-1β-purified cytokine (MIP-1β) for 24 h, followed by fixation, staining with Alexa fluor 488 phalloidin (Green) and mounting in aqueous media containing DAPI (Blue). Compared to Scr control MYO3A-directed siRNA, MDA-MB-231 cancer cells did not show any effect of MIP-1β-purified cytokine on focal degradation of pericellular matrix. Bars represent mean invadopodia count/cell from 10 fields per experiment ±SE (*p < 0.05.). All the experiments were done in triplicates and repeated at least thrice. Abbreviations—MIP-1β: Respective cancer cells treated with MIP-1β-purified cytokine.