Figure 2.
In vivo biodistribution of the Chi-Tn mAb. (A) Nude mice were grafted s.c. with 4 × 106 Shin-3 tumor cells, and were injected i.p. on day 12 with the Chi-Tn mAb or the control mAb at 20 mg/kg. On day 14, solid tumor and organs were removed and sectioned for immunofluorescence (IF) studies. (B) To detect Tn-positive cells, tissue sections were labeled with the Chi-Tn mAb, and with GaH-Fc-Biot and Sa-Cy3. (C) and (D) To detect the presence of the Chi-Tn mAb (or control mAb, red), tumor (C) or tissues (D) sections were labeled directly with GaH-Fc-Biot and Sa-Cy3. Nuclei were labeled using DAPI (blue), and images were acquired by microscopy.