Table 2.
Protocol | Number of patients | Method of antigen loading | Route of administration | IL-2a | Cisplatin |
---|---|---|---|---|---|
1 | 1 | Peptide: class I modb | i.n. | no | no |
2A | 10 | Peptide: class I wtc | i.d. | yes | no |
2B | 8 | Peptide: class I wtc | i.n. | yes | no |
2C | 10 | Peptide: class I wtc | i.n. | no | no |
2D | 10 | Peptide: class I wtc | i.d. | no | no |
2E | 10 | mRNAd | i.n. | no | no |
4A | 4 | Peptide: class I wtc + IIe | i.n. | no | no |
4B | 3 | Peptide: class I wtc | i.n. | no | no |
4C + D | 16 | mRNAd | i.n. | no | no |
5A | 5 | mRNAd | i.v./i.d. | no | no |
8A + B | 4 | mRNAd | i.n. | no | no |
9C | 9 | mRNAd | i.v./i.d. | no | yesf |
9D | 7 | mRNAd | i.v./i.d. | no | no |
Total | 97 |
Low-dose IL-2 (9 MIU) was administered subcutaneously once daily for 1 week starting 3 d after each vaccination.
Class I mod; HLA class I-restricted modified gp100-derived peptides 154–162 Q→A and 280–288 A→V and HLA class I-restricted tyrosinase-derived peptide 369–377.
Class I wt; HLA class I-restricted wild-type gp100-derived peptides 154–162 and 280–288 and HLA class I-restricted tyrosinase-derived peptide 369–377.
mRNA; mRNA encoding full length gp100 and tyrosinase.
Class II; HLA class II-restricted gp100-derived peptide 44–59 and tyrosinase-derived peptide 448–462 analog.
Cisplatin (50 mg/m2) was administered intravenously before each vaccination.
Abbreviations: wt, wild type; mod, modified; i.d., intradermal; i.n., intranodal; i.v., intravenous; IL-2, Interleukin-2.